The Mondo Liquid Culture product appears to effectively double the colonization rate of spores. You only need to inject 1 ml of spores into the vial and shake it daily. Within 2 to 14 days, you can observe the growth of the mycelium. A major advantage is that a sterile environment is not necessary for this process. Moreover, the product has a shelf life of up to a year at a temperature between 4 and 6°C. This makes it an ideal solution for mass production, preservation and culture trading. In other words, Mondo Liquid Culture offers a simple and efficient method for growing and maintaining fungal cultures.

Steps for using a Liquid Culture Spore Bottle:

1. Preparation:
   • Ensure that all materials and surfaces are clean and sterile.
   • A pressure cloth or alcohol can be used to disinfect surfaces and hands.
2. Inoculation:
   • Add spores to the liquid in the vial. This can be done using a sterile syringe.
   • Seal the bottle tightly to prevent contamination.
3. Incubation:
   • Place the vial in a warm, dark place (ideally between 21-27°C) to allow the mycelium to grow.
   • Shake the vial daily to promote mycelium growth and prevent mycelium clumping.
4. Usage:
   • Once the mycelium has grown well (this may take several days to weeks), the liquid can be used to inoculate substrate.
   • Use a sterile syringe to aspirate an amount of the liquid culture and inject it into the sterile substrate.

Benefits of Liquid Culture:

• Faster growth: Mycelium in liquid tends to grow faster than on solid substrate.
• Easy to multiply: A small amount of liquid culture can be used to inoculate several substrate pots.
• Less chance of contamination: Because the liquid is sterile, there is less chance of contamination by other microorganisms than other methods.

Contents of your Mondo Liquid Culture Vial Spore bottle

• Mondo Liquid Culture Vial 25ml
• Sterile alcohol wipes
• Sterile syringe with 18 gauge needle

Storing your Mondo Liquid Culture Vial Spore Bottle

Since this is a sensitive product, we recommend placing the spore bottle in the refrigerator immediately after receipt for 24 hours with temprature between 2 and 8 degrees before using it.